The Jim Goldie-trained Euchen Glen stayed on well to take the long-distance Dubai Duty Free Shergar Cup Stayers from Ian Williams' Byron Flyer.
Euchen Glen, a 7/1 chance, won by a neck under Dutch jockey Adrie de Vries.
Adrie de Vries drives Euchen Glen to victory, photo Liesl King
Goldie said: "The tactics worked perfectly - to switch him off in last and put him to sleep. He's a nice horse."
Asked if he had ever come across jockey de Vries before, Goldie laughed and replied: "At least the instructions - to drop him in last - were easy for to translate! It's a tactic that works well here. The one who is galloping at the finish usually wins here. It's a stiff track, especially all the way up from Swinley Bottom.
"You need a nice horse to get into this meeting. It's a long way from Scotland, but some of the jockeys have come a lot further and it has certainly justified the trip. It's a unique day, but Ascot's hospitality is second to none and whatever happens it is a good day out."
Dutch jockey Adrie De Vries, returned to the winner's enclosure draped in his country's flag.
"It was good tactics from the trainer; it was his wish [to settle the horse out the back of the field] and it was the right one," said De Vries, a veteran of two Shergar Cups at Ascot, but his first winner at the meeting.
"I wasn't expecting it so early today - I thought my best rides were at the end of the day. This is my second win around Ascot. It's a brilliant place and today is a nice day. We will make the best of it. We have some nice runs to come."
German jockey Alexander Pietsch held the inside rail for much of the race, just missing out by a neck on the Ian Williams-trained Byron Flyer.
"For the Europe team, to get the first points was good but to get the winner and second here is beautiful," said Pietsch.
In fifth, picking up a further three points for the Europe team was Umberto Rispoli on Dr Marwan Koukash's Angel Gabrial, trained in Malton by Richard Fahey.
"I got a nice race; we wouldn't have won but should probably have finished third and fourth for sure, but my horse ran an honest race," said Rispoli.
Euchen Glen, photo Liesl King
1.40pm £45,000 Dubai Duty Free Shergar Cup Stayers 2m
For 4yo+, rated 81-100 (also open to such horses rated 80 and below). Lowest weight 8st 9lb; highest weight 10st. Penalties, after August 5, 2017, for each race won 3lb.
1) EUCHEN GLEN 4-9-06 Jim Goldie  Adrie De Vries EUR 7/1 15 POINTS
2) BYRON FLYER 6-9-09 Ian Williams  Alexander Pietsch EUR 7/1 10 POINTS
3) GRACELAND (FR) 5-9-08 Michael Bell  Keita Tosaki ROW 9/2 7 POINTS
4) FLEETING VISIT 4-9-01 Eve Johnson Houghton  Emma-Jayne Wilson GIRLS 6/1 5 POINTS
5) ANGEL GABRIAL (IRE) 8-9-02 Richard Fahey  Umberto Rispoli EUR 3 POINTS
6) GAVLAR 6-9-07 William Knight  Kerrin McEvoy ROW
7) WOLFCATCHER (IRE) 5-9-03 Ian Williams  Hayley Turner GIRLS
8) COSMELLI (ITY) 4-10-00 Gay Kelleway  Anthony Delpech ROW
9) SWASHBUCKLE 4-9-08 Andrew Balding  Neil Callan GBI
10) BERRAHRI (IRE) 6-9-02 John Best  Jamie Spencer GBI
Time 3m 37.00s
Distances: nk, ¾, nk, nk, 3½
Breeder: W M Johnstone
Breeding: b g Authorized (IRE) - Jabbara (IRE) (Kingmambo (USA))
Tote Win: £8.40 Places:£2.60, £2.60, £1.60 Exacta:£58.80