Miss Temple City, who bids to make it third time lucky at Royal Ascot next week, will take on the boys in the Queen Anne Stakes on Tuesday, rather than sticking to her own sex in Wednesday's Duke of Cambridge, for which she is a shorter price.
Trained by Graham Motion, Miss Temple City can be backed at 33-1 with Ladbrokes, Coral and William Hill for the Queen Anne but is as low as 14-1 for the Duke of Cambridge, which she was fourth in last year, having finished in the same position in the Coronation Stakes in 2015.
She has been based in Lambourn at Oliver Sherwood's yard and has delighted work rider Alice Clapham with her wellbeing – something Motion agreed with when he saw her on Saturday morning.
"I'm really happy," he said. "She looks like she's right at home here at Oliver's. The weather is beautiful and she had a little four-furlong blowout this morning. It was a nice easy breeze, and I think she looks great – I couldn't be happier with her.
He continued: "One concern is she hasn't run this year, but she does go well fresh. Another concern is coming over here on what is a more testing track and making sure her fitness is at a good level, but she's been ready to run since our Derby day, which is the first week in May, so I feel pretty strongly she's fit enough, and she handled that work this morning really well."
Motion won the Kentucky Derby and Dubai World Cup with Animal Kingdom, though he was to end his career with a bitterly disappointing 11th-placed finish when favourite for the 2013 Queen Anne.
Asked about Miss Temple City's prospects, Motion added: "I think she's a bigger, stronger filly this year and her workouts at home have been impressive. I give her a slightly better chance than English bookmakers do."
Edgar Prado rides Miss Temple City, who is due to have the likes of Ribchester, Lightning Spear and fellow US-trained horse American Patriot among her rivals.